Doctoral Positions in Germany
PhD: Rotating Coherent Scattering Microscopy using absorption phase delays • Universität Freiburg • Freiburg
|Time Span||as soon as possible for 3 years|
|Application Deadline||31 Mar 2019|
|Type of Position||
|Field of Research||
|Subjects||Bio- and Nano-Photonics|
|Description||Open PhD: Rotating Coherent Scattering Microscopy using absorption phase delays
ROCS is a novel optical microscopy concept, where objects are illuminated coherently with rotating laser beams and images are generated from back-scattered coherent light. By exploiting defined multiple interferences and speckles, k-spectral shifting from oblique illumination and angular integration of many coherent images  within milliseconds, a spatial resolution of nearly 120nm can be achieved, at a temporal resolution of ≥ 100 Hz and with excellent image contrast. Our novel, rotating coherently scattered (ROCS) technique used in TIR-dark-field mode allows to acquire thousands of images without loss in image quality (e.g. fluorophore bleaching), without image reconstruction, is hence used to observe highly dynamic processes in life-sciences.
In a project funded by the DFG (by a 65% E13 position), we first want to reach a new imaging record with 120 nm spatial and 100 Hz temporal resolution. Second, we want to distinguish specifically marked structures in the image through specific absorption and phase retardation of the scattered laser light by the reference wave in brightfield mode - first at nanometer-sized particles and then at cellular structures and living cells.
We are a young and motivated team with a strong background in microscopy, in optical trapping & tracking and in biophysics. We are looking forward to answering your questions!
 Ruh,…, Rohrbach, "Superior contrast and resolution by image formation in rotating coherent scattering (ROCS) microscopy," Optica 5, 1371-1381 (2018).
|Requirements||We are seeking a motivated candidate with a strong background in optics and microscopy. The candidate should setup a new multi-laser ROCS system, perform advanced experiments & theory & computer simulations to better understand partially coherent interferences/speckles and image formation of correlated and uncorrelated photons propagating through complex media.|
|Language of Dissertation||
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